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HPLC determination of purines and pyrimidines in the products from oxidation of DNA with nitrous acid
Summary
Type:
HPLC
Application #:
107050
Detection:
UV, 260nm
Conditions:
eluent A: water, eluent B: 0.2 M ammonium formate pH 5, 4min 99.9% A, then gradient to 40% B in 4min, 10min 40% B
flow rate 1.5ml/min
Authors:
Moltrasio D
Source:
HRC 14 (1991) 777 - 779
Details
Legend: Fig. 1. HPLC chromatogram of bases resulting from oxidation of a nucleic acid with nitrous acid for 6h, 1. uracil, 2. xanthine, 3. thymine, 4. hypoxanthine, 5. guanine, 6. cytosine, 7. adenine.
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